Evaluation of the Antimicrobial, Antioxidant, and Anti-inflammatory Properties of Mukia maderaspatana Hydroalcoholic Extract

Abstract

Mukia maderaspatana, a medicinal plant with significant ethno pharmacological relevance, was investigated for its anti-inflammatory, antioxidative, and antihyperlipidemic properties. Soxhlet-assisted hot extraction and cold maceration were employed to obtain bioactive-rich fractions. Phytochemical characterization was performed using advanced analytical techniques, including Gas Chromatography-Mass Spectrometry (GC-MS) and Liquid Chromatography-Mass Spectrometry (LC-MS) for compound identification, Fourier Transform Infrared Spectroscopy (FTIR) for functional group analysis, Ultraviolet-Visible Spectroscopy (UV-Vis) for electronic transitions, and Mass Spectrometry for molecular weight determination. The most promising bioactive compounds were identified based on spectral interpretation and their potential pharmacological relevance. The extracts were subjected to in vitro evaluations to determine their efficacy in modulating inflammatory mediators, and regulating lipid metabolism. The anti-inflammatory potential was assessed through inhibition of key pro-inflammatory markers, while antioxidative activity was determined. The antihyperlipidemic effect was evaluated based on lipid profile modulation. The results demonstrated significant therapeutic potential, supporting the plant’s role in disease intervention. This study highlights the pharmacological significance of Mukia maderaspatana as a natural therapeutic agent, emphasizing its potential for further drug development. The novelty of this research lies in the identification of its bioactive constituents and their targeted pharmacological effects, reinforcing its role in herbal medicine as a viable candidate for managing inflammatory, oxidative, and lipid-associated disorders.